Influence of STAT1 on proliferation and IFN-βsensitivity of human non-small-cell lung cancer H1299 cells
10.3969/j.issn.1000-4718.2015.05.015
- VernacularTitle:STAT1对人非小细胞肺癌 H1299细胞增殖及 IF N-β敏感性的影响
- Author:
Jialu ZHAO
;
Xiaoru SUN
;
Dongxiang JI
;
Junjie CHEN
;
Mengyi WANG
;
Lei JIANG
;
Yuping LI
;
Chengshui CHEN
- Publication Type:Journal Article
- Keywords:
Signal transducer and activator of transcription 1;
H1299 cells;
Interferon-β;
Lung neoplasms
- From:
Chinese Journal of Pathophysiology
2015;(5):852-856
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of signal transducer and activator of transcription 1 ( STAT 1 ) on proliferation and interferon-β(IFN-β) sensitivity of human non-small-cell lung cancer H1299 cells.METHODS:STAT1 or EGFP gene was transfected into H1299 cells by the lentiviral vectors system.The cell number was counted under a mi-croscope and cell proliferation was tested by MTT assay.In addition, the cells transfected with STAT1 and EGFP were trea-ted with IFN-βand cell viability was measured by MTT assay.The protein levels of p-STAT1, ICAM-1 and PCNA were de-tected by Western blot.RESULTS: Over-expression of STAT1 inhibited H1299 cell proliferation (P<0.05).H1299 cells transfected with STAT1 gene had a higher sensitivity to IFN-βthan the control cells transfected with EGFP ( P <0.05).Overexpression of STAT1 increased the protein level of p-STAT1, and reduced IACM-1 expression in H1299 cells. Moreover, STAT1 enhanced STAT1 phosphorylation and downregulated the expression of PCNA in H1299 cells treated with IFN-β.CONCLUSION:STAT1 inhibits the proliferation and enhances the IFN-βsensitivity of non-small-cell lung cancer H1299 cells.
