Effects of P2X4 receptor in spinal microglia on rrTNF-induced pathologi-cal pain
10.3969/j.issn.1000-4718.2015.05.012
- VernacularTitle:脊髓小胶质细胞 P2 X4受体在 rrTNF 诱导的病理性疼痛中的作用
- Author:
Qingjuan GONG
;
Honghua WANG
;
Ying LIANG
;
Zhenhe LU
;
Jinsheng CHEN
;
Qiaodong HUANG
;
Yu YUE
- Publication Type:Journal Article
- Keywords:
P2X4 receptor;
Tumor necrosis factor-alpha;
Pathological pain;
Spinal dorsal horn;
Microglia
- From:
Chinese Journal of Pathophysiology
2015;(5):834-838
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of P2X4 receptor on peri-sciatic administration of recombinant rat TNF-α(rrTNF)-induced mechanical allodynia.METHODS:Male Sprague-Dawley rats (180~200 g) were used in the experiments.The levels of P2X4 receptor on day 3, day 7 and day 14 after peri-sciatic administration of rrTNF were exam-ined by Western blot, and the location of P2X4 receptor in the spinal dorsal horn was observed by double immunofluores-cence staining.The changes of 50%paw-withdrawal thresholds of the rat were detected by behavioral test, and the level of TNF-αin the spinal dorsal horn was also examined by Western blot when TNP-ATP was intrathecally injected before the ad-ministration of rrTNF.RESULTS:Compared with control group, the expression of P2X4 receptor in the spinal dorsal horn on the ipsilateral side significantly increased on day 3, day 7 and day 14 (P<0.01) after rrTNF (100 ng/L) administra-tion.P2X4 receptor was co-localized only with microglia, but not with neurons or astrocytes.Intrathecal injection of TNP-ATP before rrTNF administration prevented mechanical allodynia induced by rrTNF and inhibited the upregulation of TNF-αin the spinal dorsal horn.CONCLUSION:P2X4 receptors in microglia may be involved in rrTNF-induced mechanical allodynia by the upregulation of TNF-αin the spinal dorsal horn.
