Effect of Wip1 gene silencing on chemotherapy sensitivity of human colon cancer cells
10.3969/j.issn.1000-4718.2015.05.016
- VernacularTitle:W ip1基因沉默对结肠癌细胞化疗敏感性的影响
- Author:
Di WU
;
Zhongsheng XIA
;
Wa ZHONG
;
Xiji LU
;
Tao YU
;
Qikui CHEN
- Publication Type:Journal Article
- Keywords:
Colon cancer;
Small interfering RNA;
Wip1 gene;
Chemotherapy sensitivity
- From:
Chinese Journal of Pathophysiology
2015;(5):857-863
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the inhibitory effect of siRNA targeting to Wip1 gene on the Wip1 gene expression in the colon cancer cells and to investigate the influence of Wip1 gene silencing on the chemotherapy sensitivity of colon cancer cells.METHODS:Wip1-811 siRNA targeting to Wip1 gene was transfected into RKO colon cancer cells with high expression of Wip1 gene.The mRNA expression of Wip1 was measured by real-time PCR.The protein level of Wip1 was detected by Western blotting.The viability of RKO colon cancer cells was measured by MTS assay.The cell apoptosis and cell cycle were analyzed by flow cytometry.RESULTS: Wip1-811 siRNA efficiently inhibited the expression of Wip1 at mRNA and protein levels.The enhanced chemotherapy sensitivity of RKO colon cancer cells was observed after inhibition of Wip1 gene expression.The viability of RKO colon cancer cells was decreased from (89.4 ±6.6)%to (74.7 ±3.9)%af-ter treated with 5-fluorouracil (P<0.05) and decreased from (77.9 ±2.4)%to (66.7 ±2.9)%after treated with oxali-platin ( P<0.05 ) .The cell apoptotic rate was increased from ( 7.7 ±0.5 )% to ( 12.3 ±3.2 )% and from ( 14.7 ± 2.1)% to (34.0 ±2.1)% when RKO colon cancer cells were treated with 5-fluorouracil and oxaliplatin, respectively (P<0.05).CONCLUSION:Wip1 gene silencing enhances chemotherapy sensitivity of colon cancer cells.
