Platelet-rich plasma accelerates the proliferation of bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.14.003
- VernacularTitle:富血小板血浆促进骨髓间充质干细胞的增殖
- Author:
Xiang XU
;
Heping YIN
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cells;
Platelet-Rich Plasma;
Cell Proliferation;
Goats
- From:
Chinese Journal of Tissue Engineering Research
2015;(14):2144-2148
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Platelet-rich plasma contains a variety of stimulating factors, and can also raise the proteoglycan and col agen synthesis. OBJECTIVE:To observe the effect of platelet-rich plasma on the proliferation of goat bone marrow mesenchymal stem cel s. METHODS:Blood samples were extracted from the jugular vein of Inner Mongolia Ximeng goats to harvest platelet-rich plasma using centrifugation method. Then, bone marrow was extracted from the goat’s ilium by puncture method to isolate and purify goat bone marrow mesechymal stem cel s using density gradient centrifugation method. After that, primary cel s at good state were cultured in L-DMEM complete medium containing 10%, 20%, 30%platelet-rich plasma or in simple L-DMEM complete medium. RESULTS AND CONCLUSION:Within 2-6 days of culture, cel s in the platelet-rich plasma groups proliferated faster than those in the control group, and with the increasing of platelet-rich plasma concentration, the cel s grew faster, with larger number and more mature morphology. At 4 days of culture, the cel doubling time was about 50, 35, 25 hours in the 10%, 20%, and 30%platelet-rich plasma groups, respectively. These findings indicate that goat platelet-rich plasma can dramatical y promote the proliferation of bone marrow mesenchymal stem cel s in a concentration-dependent manner.
