Mycoplasma contamination decreases 28S and 18S RNC-rRNA via p38 MAPK pathways in human cell lines
10.3969/j.issn.1000-4718.2015.05.026
- VernacularTitle:支原体污染经 p38 MAPK 途径降低人类细胞株28S 和18SRNC-rRNA
- Author:
Qing WANG
;
Yanzhang LUO
;
Xiuxian LIN
;
Dongxiu RONG
;
Tao ZHANG
;
Tong WANG
;
Yizhi CUI
- Publication Type:Journal Article
- Keywords:
RNC-rRNA;
Mycoplasma;
Human cell lines
- From:
Chinese Journal of Pathophysiology
2015;(5):917-923
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the effect of mycoplasma on the rRNA composition of mature ribosomes in human cell lines.METHODS:We isolated ribosome nascent-chain complex ( RNC) from multiple mycoplasma-positive-negative human cell lines.RNC-RNA was acquired from each cell line through RNA extraction, followed by agarose gel separation, fluorescent visualization and gray-scale value measurements on the rRNA bands.Western blot analysis was performed to in-vestigate the MAPK pathway alterations.RESULTS:In various human cell lines derived from different tissues, we found that as compared with mycoplasma-negative cells, mycoplasma-positive cells showed aberrant RNC-rRNA band patterns, featured by the decreases in 28S and 18S eukaryotic rRNAs and the increases in 16S and other unknown prokaryotic rRNAs.When cultured without ciprofloxacin, mycoplasma-negative HBE cells acquired mycoplasma contamination as ob-served with such characteristic abnormal rRNA bands.The ciprofloxacin treatment was able to recover the RNC-rRNA bands of the mycoplasma-contaminated cells to the normal pattern.The results of Western blot analysis on total and phos-phorylated proteins indicated that p38 pathway was significantly deactivated in mycoplasma-infected A549 cells, while ERK1/2 pathway was not significantly altered.CONCLUSION:Mycoplasma contamination severely alters the RNC-rRNA composition via p38 MAPK pathway, thus seriously impacting on the host cell translational behaviors.
