Effect of ABCE1 gene silencing on proliferation and migration of human bladder cancer cell line T24
10.3969/j.issn.1000-4718.2015.05.028
- VernacularTitle:ABCE1基因沉默对人膀胱癌 T24细胞增殖和迁移能力的影响
- Author:
Qiang LI
;
Jiujiang WANG
;
Shuifa LUO
;
Junjie ZHU
- Publication Type:Journal Article
- Keywords:
Bladder cancer;
ABCE1 gene;
T24 cells;
Cell proliferation
- From:
Chinese Journal of Pathophysiology
2015;(5):929-933
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of small interfering RNA ( siRNA)-mediated ABCE1 knockdown on the survival, cell cycle and invasion of human bladder cancer cell line T24.METHODS:The siRNA against ABCE1 was constructed and transfected into the T24 cells with LipofectamineTM 2000.The expression of ABCE1 was detected by RT-PCR and Western blot.Flow cytometry was used to detect the cell cycle.The effects of ABCE1 gene silencing on prolifera-tion, migration and invasion of T24 cells were evaluated by CCK-8 assay, wound-healing assay and Transwell invasion as-say, respectively.RESULTS: Compared with control group and blank group, the mRNA and protein levels of ABCE1 were significantly decreased in experimental group after transfected with ABCE1 siRNA.The cell cycle was arrested at G0/G1 phase and the cell number in S phase was decreased in the T24 cells.Compared with control group and blank group, the proliferation of T24 cells in experimental group was inhibited significantly, and the migration and invasion abilities of T24 cells in experimental group decreased significantly.CONCLUSION:Knockdown of ABCE1 gene may decrease migration, invasion and proliferation abilities in T24 cells.
