Screening of Tiam1-related genes contributing to metastasis of colorectal cancer using a gene expression profiling chip
10.13315/j.cnki.cjcep.2015.04.002
- VernacularTitle:表达谱基因芯片筛选结直肠癌转移中Tiam1相关基因
- Author:
Yu XIAO
;
Lina YU
;
Yanqing DING
- Publication Type:Journal Article
- Keywords:
colorectal neoplasm;
cancer metastasis;
Tiam1;
gene chip;
PI3K/AKT/mTOR signaling pathway
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(4):363-367
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To screen and identify T cell lymphoma invasion and metastasis 1 (Tiam1) related genes and expression profile during distant metastasis of colorectal cancer. Methods Colorectal cancer( CRC) in a mice model was established by intraperitoneal injection of dimethylhydrazine ( DMH) . Five panels of fresh primary tumor tissues from Tiam1 transgenic mice were collected and com-pared with that from wild type mice. Differentially expressed genes were detected by Affymetrix human genome-wide expression profile chip and verified by real-time quantitative PCR ( qRT-PCR) analysis. Results The genechip result showed that 794 genes were differ-entially expressed by at least 3 folds in Tiam1 transgenic mice with distant metastasis of colorectal cancer tissue, including 400 up-regu-lated and 394 down-regulated ones. Bioinformatics analysis and gene co-expression network building identified 3 genes (PIK3R5, FIGF and RPS6KA6) with specific expression in colorectal cancer tissue with distant metastasis, and this result was confirmed by qRT-PCR. Conclusion A specific Tiam1 gene expression profile related to colorectal cancer distant metastasis has been established through screening and identifying the genes related to Tiam1 by gene chip technique. These findings provide a basis for exploring the molecular markers of colorectal cancer with distant metastasis.
