Screening of hypertension/blood stasis syndrome-related miRNA in endo-thelial cell models
10.3969/j.issn.1000-4718.2015.05.009
- VernacularTitle:高血压病血瘀证相关 m iRNA 的筛选
- Author:
Ling HE
;
Meixia FANG
;
Liguo CHEN
;
Jing YUAN
;
Jianhua ZHOU
;
Jing XU
- Publication Type:Journal Article
- Keywords:
Hypertension;
Blood stasis syndrome;
MicroRNA
- From:
Chinese Journal of Pathophysiology
2015;(5):817-822
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To screen the miRNA related to blood stasis syndrome and to explore the genetic mechanism of blood stasis syndrome in hypertension.METHODS:Human umbilical vein endothelial cell line CRL-1730 was co-cul-tured with the human sera from healthy normal controls and hypertension patients to establish a blood stasis-hypertension cell modela.The hypertension patients showed qi deficiency and blood stasis, qi stagnation and blood stasis, cold retaining and blood stasis, heat retaining and blood stasis and non-blood stasis syndromes.The endothelial cell models of blood stasis in hypertension were established.Target cells were collected for total RNA extraction.The techniques of Solexa ( high-throughput sequencing method) and digital gene expression profiling were applied for screening the target miRNA, and miRWalk software was used for online prediction the mRNA which might be the relevant target.qRT-PCR was conducted to confirm the prediction.RESULTS:SAT1-Hsa-miR-199a-5p and ATF4-Hsa-miR-1283 were found to be highly expressed in these cell models.The expression of miR-1283 and miR-199a-5p was confirmed by qRT-PCR.CONCLUSION:miR-199a-5p and miR-1283 may be the relevant miRNA for the prediction of blood stasis syndrome in hypertension.
