Resveratrol protects oxygen-glucose deprivation rat cortical neurons by regulating SIRT1/AMPK signaling pathways
10.3760/cma.j.issn.1673-4165.2015.03.008
- VernacularTitle:白藜芦醇通过调节 SIRT1/AMPK 信号通路保护氧-葡萄糖剥夺大鼠皮质神经元
- Author:
Yongjiu WANG
;
Zhenqing GONG
;
Zhihui WANG
;
Yan CHENG
- Publication Type:Journal Article
- Keywords:
Neurons;
Cels,Cultured;
Oxygen;
Glucose;
Stilbenes;
Sirtuin 1;
AMP-Activated Protein Kinases;
Neuroprotective Agents;
Rats;
Resveratrol
- From:
International Journal of Cerebrovascular Diseases
2015;(3):193-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of resveratrol on the first and double oxygen-glucose deprivation (OGD) primary cortical neuron silent information regulator 1 (SIRT1), AMP-activated protein kinase (AMPK) activity and ATP content, and its possible neuroprotective mechanism. Methods Cortical neurons were taken from the embryos of 18-day Wistar rats. An in vitro repeated ischemia model was induced by the double OGD after the success of primary culture. Trypan blue stalning was used to detect the cel survival rate. Western blot was used to detect the SIRT1 and phospho-AMPK expression. Deacetylase fluorescence assay was used to detect the SIRT1 activity. Bioluminescence assay was used to detect the ATP content. Results Compared with the control group, resveratrol (0. 5 μmol/L) preconditioning significantly increased the survival rates after the single and double OGD (al P < 0. 001), ATP content (al P = 0. 004), SIRT1 activity (single: P = 0. 001; double: P = 0. 002), and the expression levels of SIRT1 (single: P = 0. 029; double: P = 0. 023) and phospho-AMPK (al P = 0. 001). Conclusions Resveratrol has the neuroprotective effect for the first and double OGD cortical neurons. Its mechanism may be associated with upregulating the SIRT1/AMPK signaling pathways and decreasing the energy requirements.
