Simultaneous Determination of Rutin, Hyperin and Quercetin in Vicia Sepium L. by RP-HPLC
- VernacularTitle:HPLC法同时测定野豌豆中芦丁、金丝桃苷和槲皮素的含量
- Author:
Qingjie CHEN
;
Jie YANG
;
Luzhen GAN
;
Ninghua WU
- Publication Type:Journal Article
- Keywords:
Vicia sepium L.;
HPLC;
Quality control;
Flavonoid
- From:
China Pharmacist
2015;(5):861-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC method for the determination of rutin, hyperin and quercetin in Vicia sepium L. . Methods:The samples were separated on an Agilent ZORBAX Eclipse XDB-C18 column(250 mm × 4. 6 mm, 5 μm) with the mobile phase consisting of acetonitrile-1‰ phosphoric acid solution with gradient elution at the flow rate of 0. 8 ml·min-1 . The column tem-perature was 30℃, and the detection wavelength was set at 370 nm. Results: The linear range of the three components was 4. 090-130.940 μg ·ml-1(r=0.999 9), 4.600-147.200 μg ·ml-1(r=0.999 9) and 0.810-25.780 μg·ml-1(r=0.999 8), and the average recovery was 103. 45% (RSD=1. 25%), 98. 96% (RSD=1. 77%) and 102. 88% (RSD=0. 84%)(n=6), respectively. Conclusion:The method is stable, reproducible and simple, which can be used in the quality control of Vicia sepium L. .
