A profile of cross-reactive neutralizing antibodies against various subtypes of HIV-1 strains in people with HIV-1 infection in Chongqing city
10.3760/cma.j.issn.0254-5101.2015.03.003
- VernacularTitle:重庆市 HIV-1慢性感染者亚型交叉反应中和抗体的分析
- Author:
Yi LIN
;
Quanhua ZHOU
;
Min ZHANG
;
Tao WANG
;
Fangwei SHEN
;
Yile XUE
;
Ying WANG
;
Ping ZHONG
- Publication Type:Journal Article
- Keywords:
HIV-1;
Broadly neutralizing antibodies
- From:Chinese Journal of Microbiology and Immunology
2015;(3):167-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the levels of cross-reactive neutralizing antibodies responding to various subtypes of HIV-1 strains in people with HIV-1 infection in Chongqing and to analyze their character-istics.Methods Two hundred and thirty-six plasma samples were collected from patients with chronic HIV-1 infection in Chongqing city.The single-round-infection assay in TZM-bl cells were performed to screen the plasma samples with cross-reactive neutralizing antibodies against various subtypes of HIV-1 strains by using HIV-1 pseudovirus strains and to measure the 50%inhibitory dose ( ID50 ) .Results Eight-een out of 236 (7.63%) plasma samples were able to neutralize various B subtypes and/or C subtypes of HIV-1 pseudovirus strains, among which 15 plasma samples showed the great ability to neutralize pseudovir-us strains of different subtypes.Conclusion The cross-reactive neutralizing antibodies against various sub-types of HIV-1 strains existed in plasma samples collected from people with chronic HIV-1 infection in Chongqing city.
