Intervention of AMD3100 in lung tissues of rats during pulmonary oxygen intoxication
10.7644/j.issn.1674-9960.2015.04.003
- VernacularTitle:CXCR4抑制剂对大鼠肺型氧中毒肺损伤的干预作用
- Author:
Shi ZHANG
;
Yiqun FANG
;
Pu YOU
;
Jian YAO
;
Kaicheng LI
;
Xiaochen BAO
- Publication Type:Journal Article
- Keywords:
chemokine receptor 4;
AMD3100;
oxygen intoxication;
lung tissues;
rat
- From:
Military Medical Sciences
2015;(4):250-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intervention of chemokine receptor 4(CXCR4) antagonist AMD3100 in lung tissues of rats during pulmonary oxygen intoxication.Methods Forty SD rats were randomly divided into 4 groups:normal pressure air PBS group, normal pressure air antagonist group , oxygen exposure PBS group and oxygen exposure antagonist group, each consisting of 10 animals.The last two groups were compressed to 0.23 MPa at an exponential rate of 0.1 MPa/min by pure oxygen.Pathological changes of lung tissues were observed by hematoxylin eosin stain.Changes in TNF-αand IL-1βexpression levels in the lung tissues of rats were detected by ELISA.Changes in CXCR4 expression levels were ob-served by Western blotting.Results Pathological examination indicated that edema and hemorrhage in the alveolar and pulmonary interstitial tissue of oxygen exposure antagonist group were lighter than in oxygen exposure PBS group.The levels of TNF-α, IL-1βand cleaved-caspase-3 in the lung tissues of the oxygen exposure antagonist group were lower than in oxy-gen exposure PBS group.Conclusion Blocking CXCR4 with AMD3100 can effectively alleviate lung injury during pulmo-nary oxygen intoxication.
