Effect on Distribution and Expression ofμ-opioid Receptor in Hippocampus of Premenstrual Syndrome Liver-qi Stagnation Rat Model by Shu-Yu Capsule
10.11842/wst.2015.04.006
- VernacularTitle:舒郁胶囊对PMS肝气郁证模型大鼠海马μ阿片受体分布表达的影响
- Author:
Meiyan WANG
;
Chunhong SONG
;
Ling XUE
- Publication Type:Journal Article
- Keywords:
Shu-Yu capsule;
premenstrual syndrome liver-qi stagnation;
rat;
μ-opioid receptor
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;17(4):782-787
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to discuss the distribution and protein expression level ofμ-opioid receptor (MOR) in hippocampus of premenstrual syndrome (PMS) liver-qi stagnation rat model, in order to initially reveal the action mechanism of PMS liver-qi stagnation and intervention effect ofShu-Yu (SY) capsule. Chronic restraint stress method was used to copy PMS liver-qi stagnation rat model.SY capsule ofTiao-Gan prescription was given as intervention. Immunofluorescence (IF) and western blot (WB) technique were used to detect MOR in hippocampal CA1 and CA3 brain area of rats from each group. The results showed that compared with the normal group, the hippocampus MOR distribution arrangement was messy with increased protein concentration in the model group (P< 0.01). After drug intervention, the MOR protein level returned to normal level. It was concluded that the pathogenesis of PMS liver-qi stagnation may be associated with high expression of MOR in hippocampus CA1 and CA3 region of rats. SY capsule can effectively correct and restore it to nearly normal level. It may be one of the central mechanisms in SY capsule treatment of PMS liver-qi stagnation.
