Study on Bi-Min-Gan Granules for Regulation of Allergic Rhinitis Treg Cells Induced Immune Tolerance
10.11842/wst.2015.04.015
- VernacularTitle:鼻敏感颗粒调控变应性鼻炎Treg细胞诱导免疫耐受的研究
- Author:
Jun SHI
;
Yu LIU
;
Shihai YAN
;
Daonan YAN
- Publication Type:Journal Article
- Keywords:
Rhinitis;
allergic;
Bi-Min-Gan granules;
regulatory T cell;
immune tolerance
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;17(4):841-845
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to exploreBi-Min-Gan (BMG) granules induced immune tolerance for allergic rhinitis cases through changes of Treg cells to achieve the goal of treatment. SD rats were used as experimental animal, which were divided into the blank group, model group and BMG granules group. Allergic rhinitis rat model was established. Intragastric administration of BMG granules was given. Levels of CD4+CD25+Foxp3+Treg and IL-4, IL-10, TGF-β1, IL-13 were detected with flow cytometry and ELISA assay. The results of flow cytometry showed that CD4+CD25+Foxp3+Treg decreased after the successful establishment of the model (P<0.05); but it increased after treatment of BMG granules (P<0.05) to reach the level of the normal blank group (P>0.05). ELISA showed the concentrations of IL-10 and TGF-β1 decreased significantly after the successful establishment of the model (P<0.0001); concentrations of IL-4 and IL-13 increased significantly (P<0.0001). While, after treatment of BMG granules, concentrations of IL-10 and TGF-β1 in blood rebound but did not reach the level of the normal blank group;concentrations of IL-4 and IL-13 in the blood decreased but did not reach the level of the normal blank group. It was concluded that BMG granules can upregulate the number of Treg cells in allergic rhinitis rat, increase the secretion of IL-10 and TGF-β1, inhibit Th2 cell activity, and reduce secretion of IL-4 and IL-13, so as to improve symptoms of allergic rhinitis.
