Protective Effect of Catalpol on Vascular Endothelial Cell Injured by High Glucose
10.11842/wst.2015.04.016
- VernacularTitle:梓醇对高糖所致血管内皮细胞损伤的保护作用
- Author:
Hao HONG
;
Shuping FU
;
Chen OU
;
Bingmei ZHU
- Publication Type:Journal Article
- Keywords:
Vascular endothelial cell;
high glucose;
catalpol;
NO;
LDH
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;17(4):846-849
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the protective effect of catalpol against high glucose induced injury on vascular endothelial cells (ECV-304). ECV-304 cells were culturedin vitro. MTT method was applied to detect cell viability. NO and LDH level were measured by spectrophotometer method. The effect of catalpol against high glucose induced injury on ECV-304 was observed. The results showed that there were no obvious changes in cell viabilities of ECV-304 cultured in normal concentration of glucose medium among groups with different concentrations of catalpol. Compared with the control group, cell viability was significantly decreased (P< 0.01), the NO secretion was significantly reduced (P< 0.01), the LDH level was increased obviously (P< 0.01) in the high glucose (30 mmol·L-1 glucose) injury model group. The high osmotic (24.5 mmol·L-1 mannitol) group was set up as a positive control. Comparison between the low dose (1 ng·μL-1 catalpol + 30 mmol·L-1 glucose), middle dose (10 ng·μL-1 catalpol+ 30 mmol·L-1 glucose), and high glucose (30 mmol·L-1 glucose) injury model group showed no changes on cell viability and NO (P> 0.05); while the LDH level was reduced (P< 0.01). Comparison between the high dose catalpol (100 ng·μL-1 catalpol + 30 mmol·L-1 glucose) group and the high glucose model group showed that the cell viability was obviously increased (P< 0.01); NO secretion was increased (P< 0.05); and the LDH level was obviously reduced (P< 0.01). It was concluded that catalpol had protective effect against high glucose induced ECV-304 injury through the regulation of NO and LDH level. The effect of high dose was obvious.
