Effects of Pomegranate Leaf Tennis and Ellagic Acid on In Vitro Primary Rat Adipocyte Transformation and Lipolytic-related Factor Expression

VernacularTitle:石榴叶鞣质和鞣花酸体外对大鼠原代脂肪细胞转化及脂代谢相关因子表达的影响
Author: Xuan YU ; Xi LU ; Jingfei JIANG ; Xinpei WANG ; Dongming XING ; Fan LEI ; Lijun DU
Publication Type:Journal Article
Keywords: Pomegranate leaf; ellagic acid; adipocyte; lipid metabolism
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;17(3):520-525
CountryChina
Language:Chinese
Abstract: This study was aimed to observe effect of pomegranate leaf tannin and ellagic acid on primary rat adipocyte differentiation and lipid metabolism-related factor expression. Primary rat preadipocyte was in vitro cultured to observe the effect of pomegranate leaf tannin and ellagic acid on lipid fat cells as well as mRNA expression of its related factor. The results showed that pomegranate leaf tannin and ellagic acid had obvious inhibition effect on fat formation in fat cells. It had certain inhibition effect on activities of lipoprotein lipase (LPL) and glucose-3-phosphate dehydrogenase (GPDH). It promoted fat decomposition and reduced intracellular lipid content. It upregulated PPARγ and fatty acid-binding protein (aP2). It downregulated obese (ob) gene level. It was concluded that pomegranate leaf tannin can inhibit fat generation of fat cells and promote fat metabolism. Ellagic acid was its main active ingredient, which had the same effect as pomegranate leaf tannin.