Resisin stimulates the expression of CCL3 and CCL4 in chondrocytes
10.3969/j.issn.2095-4344.2015.15.001
- VernacularTitle:抵抗素刺激软骨细胞趋化因子CCL3及CCL4基因表达及机制
- Author:
Ziji ZHANG
;
Yan KANG
;
Zibo YANG
;
Changhe HOU
;
Guangxin HUANG
;
Weishen CHEN
;
Puyi SHENG
;
Aishan HE
;
Ming FU
;
Weiming LIAO
;
Zhiqi ZHANG
- Publication Type:Journal Article
- Keywords:
Subject headings:Chondrocytes;
Chemotactic Factors;
NF-kappa B
- From:
Chinese Journal of Tissue Engineering Research
2015;(15):2297-2304
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies have indicated that resistin stimulates a large set of chemokines in chondrocytes that are known to be important in inflammatory joint lesions.
OBJECTIVE:To further investigate the mechanism of co-regulation roles of transcription and post-transcription in the up-regulation of two chemokine genes CCL3 and CCL4 in chondrocytes in response to resistin.
METHODS:Human chondrocytes, T/C-28a2 and ATDC5 cels were cultured. The function of resistin on the chemokine genes, and the expression of C/EBPβ, nuclear factor-κB isoforms and chondrogenic specific miRNAs were tested by qPCR. The co-regulation of C/EBPβ and nuclear factor-κB was investigated by nuclear factor-κB inhibitor (IKK-NBD) and C/EBPβ inhibitor (SB303580) treatments, and subcelular localization was detected with or without resistin stimulation.
RESULTS AND CONCLUSION:Resistin could increase the expression of chemokine genes independently. Chondrocytes reacted in a non-restrictedly cel-specific manner to resistin; C/EBPβ inhibitor, nuclear factor-κB and some chondrogenic specific miRNAs in a combinatorial manner regulated chemokine gene expression. The activity of C/EBPβ was augmented by a transient increase in activity of nuclear factor-κB, and both transcription factors acted independently on the chemokine genes, CCL3 and CCL4.
