Topical application of nerve growth factor in early fracture healing after peripheral nerve injury
10.3969/j.issn.2095-4344.2015.15.005
- VernacularTitle:局部应用神经生长因子对周围神经损伤后骨折早期愈合的影响
- Author:
Chongxi ZHAO
;
Jun MA
;
Ning HE
;
Zhaohui CHEN
- Publication Type:Journal Article
- Keywords:
Subject headings：Nerve Growth Factor;
Fractures,Bone;
Fracture Healing
- From:
Chinese Journal of Tissue Engineering Research
2015;(15):2320-2324
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Fracture healing mechanism is complex and affected by many factors, and delayed fracture healing or nonunion often occurs. How to promote fracture healing has become a serious problem.
OBJECTIVE:To observe the effect of local application of nerve growth factor on early fracture healing after peripheral nerve injury.
METHODS:Thirty-six healthy male Wistar rats were selected to establish tibial fracture models, which were randomly divided into four groups, with 18 limbs in each group. Group A: tibial fracture+normal saline injectionvia bilateral gastrocnemius muscles; group B: tibial fracture+nerve injury+normal saline injection; group C: tibial fracture+local injection of nerve growth factor; group D: tibial fracture+nerve injury+local injection of nerve growth factor. Calus metrology results were compared among different groups.
RESULTS AND CONCLUSION:The calus volume was the most in the group B at 4 weeks of intervention, but there were no different among the other three groups (P > 0.05). At 2 weeks of intervention, the bone resorption area was significantly larger in the group B than the group D (P < 0.05), and the osteoclast index was significantly higher in the group A than the group C (P < 0.05); while at 4 weeks of intervention, the mineralized bone trabecular width was significantly lower in the group A than the group C (P < 0.05) as wel as lower in the group C than the group D (P < 0.05). These findings indicate that after peripheral nerve injury, local application of nerve growth factor can enhance the osteogenic ability, effectively inhibit osteoclast activity, and promote the early healing of fracture.
