Effect ofCoreopsis tinctoria Nutt. from Different Extract Regions on Proliferation and Differentiation of 3T3-L1 Preadipocytes
10.11842/wst.2015.03.021
- VernacularTitle:雪菊不同提取部位对3T3-L1前脂肪细胞增殖与分化的影响
- Author:
Mingxian JIA
;
Yuan ZHANG
;
Shifen DONG
;
Dai DAI
;
Jie YU
;
Wenjie GU
;
Zhiyuan LI
;
Jing WANG
- Publication Type:Journal Article
- Keywords:
Coreopsis tinctoria Nutt.;
3T3-L1 preadipocytes;
proliferation;
differentiation
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;17(3):544-549
- CountryChina
- Language:Chinese
-
Abstract:
The 3T3-L1 preadipocytes were used as carriers in the investigation of total extract, n-butanol extract, CB-1 and CB-2 of Coreopsis tinctoria Nutt. on cell proliferation and differentiation. Three groups at different doses were set for each of the four extract regions of C. tinctoria Nutt., respectively. MTT assay was used to detect 3T3-L1cell proliferation by four extract regions of C. tinctoria Nutt. Oil Red O staining was used to analyze the formation and accumulation of cytoplasmic lipid during cell differentiation. The results showed that compared with the control group, there were significant inhibition on cell proliferation when thetotal extract of C. tinctoriaNutt. at 100 μg·mL-1, n-butanol extract at 0.5, 5, and 50 μg·mL-1, CB-1 and CB-2 at 50 μg·mL-1 (P< 0.01). N-butanol extract showed certain dose-dependent manner (r = -0.903). Oil Red O staining showed that compared with the control group, thetotal extract of C. tinctoria Nutt. at 1, 10, 100 μg·mL-1 can obviously inhibit cell differentiation, reduce the formation of cytoplasmic lipid (P< 0.01). N-butanol extract can inhibit cell differentiation in a dose-dependent manner (r= -0.779). CB-1 and CB-2 obviously inhibited cell differentiation at the concentration of 50 μg·mL-1 (P < 0.01). It was concluded that thetotal extract, n-butanol extract, CB-1 and CB-2 of C. tinctoria Nutt. can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes and reduce the formation of cytoplasmic lipid.
