Role of GSK-3βactivity and microglial TLR4 receptor in POCD
- VernacularTitle:GSK-3β活性与小胶质细胞TLR4受体在POCD发生中的作用机制
- Author:
Bo ZHANG
;
Shizheng WU
;
Quanzhong HU
;
Qian HOU
;
Ding CAI
;
Yanning QIAN
- Publication Type:Journal Article
- Keywords:
postoperative cognitive dysfunction;
glycogen synthase kinase -3β;
Toll-like receptor 4;
microglia;
mechanism
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(4):39-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore glycogen synthase kinase -3β( GSK-3β) activity and Toll-like receptor 4 ( TLR4 ) proteins expression of microglia were tested in vitro experiments, and the possible mechanism of postoperative cognitive dysfunction(POCD).Methods The cell morphology of primary culture microglia was observed by inverted microscope;microglia were identified by glial fibrillary acidic protein ( GFAP ) immunofluorescence;the best POCD modeling conditions of microglia injury induced by lipopolysaccharides( LPS) were screened ; microglia vigor was assayed by MTT ; the proteins expressions of GSK-3βand TLR4 of microglia were detected by Western blot.Results GFAP immunofluorescence showed a positive result that primary culture of rat microglia was successful;MTT result showed that the best PODC modeling conditions of microglia injury induced by LPS (100 ng/mL) was 7h; Western blot results showed that the preotein expressions of GSK-3βand TLR4 of microglial cells were up-regulated by LPS compared with the control group,and there were significantly differences (P<0.01).Conclusion PODC pathogenesis may be associated with LPS that could up-regulat the protein expression of GSK-3βand TLR4 in microglial cells.
