Metabolism profile of sulfation of chrysin in human small intestine S9
- VernacularTitle:白杨素在人小肠S9中磺酸化结合反应的代谢特性
- Author:
Kunyu JIANG
;
Yiping ZHOU
;
Yinglin MA
;
Yu ZHOU
;
Maofan ZHANG
;
Shengnan MENG
- Publication Type:Journal Article
- Keywords:
chrysin;
SULT1A3;
human small intestine S9;
sulfation
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(4):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the metabolic profile of chrysin in SULT1A3 and human small intestine S9.Methods After incubation of chrysin using in vitro SULT1A3 and human small intestine S9 system, high-performance liquid chromatography was utilized to determine the sulfates of chrysin.Mass spectrum(MS) were employed to elucidate the structures of metabolite.Results In the SULT1A3 with PAPS, Km were (3.06 ±1.04) and (0.41±0.06) μM, Vmax were (12.13 ±1.30) and (6.72 ±1.61) nmol/(min· mg), Vmax/Km were 3.96 and 16.39 mL/(min· mg), respectively.In the human small intestine S9 with PAPS, Km were (1.92 ±0.35) and (0.01 ±0.00) μM, Vmax were (0.52 ±0.02) and (0.08 ± 0.02) nmol/(min· mg), Vmax/Km were 0.27 and 8.00 mL/(min· mg).The metabolic behavior of chrysin in SULT1A3 and human small intestine S9 both were followed biphasic kinetics.The sulfation of chrysin in SULT1A3 showed a significant correlation with that in human small intestine S9(R2 =0.985).Conclusion The result indicates that SULT1A3 is the major enzyme to the metabolism of chrysin, human small intestine may be the main metabolic organs of chrysin.
