Research on the determination of molecular weight distribution of anti-HBV placenta transfer factor injection
- VernacularTitle:抗乙肝胎盘转移因子注射液分子量分布的研究
- Author:
Haojun ZHANG
;
Yuxin ZHAO
;
Shengbin SUN
;
Guozhi JIANG
;
Zhengpin WANG
;
Zhong CHEN
;
Mingzheng ZHANG
- Publication Type:Journal Article
- Keywords:
anti-HBV placenta transfer factor injection;
SDS-PAGE;
HPSEC;
MALDI-TOF-MS;
molecular weight distribution
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(4):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the molecular weight distribution of anti-HBV placenta transfer factor injection (PSTF) by electrophoresis, HPLC and MS.Methods Using the methods of SDS-PAGE, HPSEC, MALDI-TOF-MS to test the molecular of PSTF.Results The Molecular was 8000 Da by SDS-PAGE.There were 5026.67,6783.44,7496.42,8736.55 Da components in PSTF by HPSEC.The main component molecular was 2972 Da and the maximum molecular component was 8194 Da.Conclusion HPSEC is simple and rapid to determine the maximum component molecular of PSTF.
