A comparative study on determination of blood drug concentration of sodium valproate in epileptic children between EMIT and HPLC
- VernacularTitle:均相酶扩大免疫分析法与高效液相色谱法测定癫痫患儿丙戊酸钠血药浓度比较研究
- Author:
Yan YANG
;
Wanting ZHANG
;
Xiaodong YAO
;
Xiaoyan GOU
- Publication Type:Journal Article
- Keywords:
enzyme-multiplied immunoassay test;
high performance liquid chromatography;
sodium valproate;
blood drug concentration
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(4):169-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation of blood drug concentration determination of sodium valproate in children with epilepsy by using enzyme-multiplied immunoassay test (EMIT) and high performance liquid chromatography (HPLC), so as to provide basis of selection in clinical determination of sodium valproate ( VPA) .Methods 200 blood samples of epileptic children taking sodium valproate were collected, blood concentration were determined by EMIT and HPLC method, and the correlation was analyzed.Results The following regression equation was obtained by determination results of HPLC (X) and EMIT (Y): Y=1063.517X-331.351(r=0.933), which had significant correlation (P<0.05).The plasma concentration of VPA determined by EMIT was higher than that by HPLC.Conclusion The determination results of VPA concentration in epileptic children by using EMIT and HPLC has significant correlation, and it should choose the suitable method to determine the blood drug concentration of VPA based on the actual situation.
