Effect and Mechanism of Cortex Lycii Radicis Extracts on the Proliferation and Apoptosis of Pancreatic Beta Cell
10.16466/j.issn1005-5509.2015.06.016
- VernacularTitle:地骨皮醇提液对胰岛β细胞增殖和凋亡的影响
- Author:
Dong WANG
;
Zhen YE
;
Qi HUANG
- Publication Type:Journal Article
- Keywords:
diabetes;
cortex Lycii Radicis;
concentration;
pancreatic beta cell;
INS-1;
cell proliferation;
apoptosis
- From:
Journal of Zhejiang Chinese Medical University
2015;(6):478-481
- CountryChina
- Language:Chinese
-
Abstract:
Objective] Observing Cortex Lycii Radicis' effect(Cortex Lycii) on rat insulinoma cells(INS-1) proliferation and apoptosis. To explore the mechanism of Cortex Lycii on Pancreatic Beta Cell Proliferation and apoptosis.[ Methods] After primary culture, cells were randomly divided into blank control group:control,11.1mmol·L-1 glucose,HG,30mmol·L-1 glucose, HG+Cortex Lycii(1g·L-1), HG+Cortex Lycii(2g·L-1), HG+Cortex Lycii(4g·L-1), the survival rate of cells was observed by cell counting kit-8(CCK-8);the apoptosis rate was observed by Annexin V stammg. [Results] ①Compared with HG the better effect of cell proliferation groups of Cortex Lycii(P<0.01), the best is group of Cortex Lycii(2g·L-1)(P<0.05) ②Compared with HG group the higher survival rate is group of Cortex Lycii(P<0.01), the lower apoptosis rate of Cortex Lycii(2g·L-1) compared with Cortex Lycii(1g·L-1). [Conclusion] Cortex Lycii can promote the proliferation of pancreatic beta cell, inhibit the apoptosis to protect the pancreatic beta cell. The optimal concentration is 2g·L-1.
