Effect of intrauterine growth retardation on gluconeogenic enzymes in rat liver
10.11817/j.issn.1672-7347.2014.04.013
- VernacularTitle:宫内发育迟缓对大鼠肝糖异生关键酶的影响
- Author:
Kaiju LUO
;
Pingyang CHEN
;
Zongde XIE
;
Wen LI
;
Suping LI
;
Mingfeng HE
- Publication Type:Journal Article
- Keywords:
intrauterine growth retardation;
insulin resistance;
liver;
gluconeogenic enzyme
- From:
Journal of Central South University(Medical Sciences)
2014;(4):395-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of gluconeogenic enzymes phosphoenolpyruvate carboxykinase (PEPCK) and G-6-Pase mRNA of hepatic tissue in rats with intrauterine growth retardation (IUGR) and to explore the molecular mechanism of insulin resistance in IUGR rats. Methods: Pregnant rats were randomly divided into 2 groups: a normal group and a model group. hTe normal group were fed with 21% protein forage and the model group with 10% low protein forage to obtain IUGR pup rats. hTe pup rats were introduced to the normal group and the IUGR group prospectively. At 1, 3 and 8 weeks, the body weight, blood glucose, insulin concentration andinsulin resistance index of the pup rats were measured. Expression of PEPCK and G-6-Pase mRNA were detected by RT-PCR. Results: The birth weight of the IUGR group was significantly lower than that of the normal group (P<0.001). The weight of the IUGR group was still lower than that of the normal group at 1, 3 and 8 weeks. There was no significant difference in the blood glucose, insulin level and insulin resistance index between the 2 groups (P>0.05). The hepatic expression of PEPCK and G-6-Pase mRNA in the IUGR group was significantly higher than that of the normal group at 1, 3 and 8 weeks (P<0.01). Conclusion: The significantly increased expression of PEPCK and G-6-Pase mRNA of hepatic tissue in IUGR rats may increase gluconeogenesis, which is probably one of the molecular mechanisms of insulin resistance and diabetes in IUGR.
