Influence of chronic cobalt chloride treatment on expression of Caspase-3 apoptosis gene within myocar-dial infarction area in GK diabetic rats
10.3969/j.issn.1008-0074.2015.03.01
- VernacularTitle:慢性氯化钴处理对Caspase-3凋亡基因在糖尿病大鼠心肌梗死区表达的影响
- Author:
Yujie WANG
;
Guoxian QI
;
Daifa HUANG
;
Xiaowei ZHENG
- Publication Type:Journal Article
- Keywords:
Caspase 3;
Diabetes mellitus;
Rats;
Apoptosis
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2015;24(3):231-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of chronic cobalt chloride (CoCl2 ) treatment on blood glucose and car-diomyocyte apoptosis in diabetic rats .Methods:In vivo MI model of rat was established using male GK diabetic rats and male Wistar rats (all weighing 200g~250g) .Blood glucose level was measured before model establishment .Af-ter successful operation ,rats were divided into six groups:Wistar sham operation group ,Wistar MI group ,Wistar MI + CoCl2 group ,GK sham operation group ,GK MI group and GK MI + CoCl2 group ,six rats survived in each group at least .After three weeks ,blood glucose level was measured and heart was excised ,expression of Caspase-3 apoptosis gene in ischemic necrosis area was measured .Results:(1) Compared with GK sham operation group and GK MI group on three weeks after operation ,there was significant reduction in blood glucose level [ (27.73 ± 2.58) mmol/L ,(27.87 ± 3.18) mmol/L vs .(16.3 ± 2.15) mmol/L] in GK MI + CoCl2 group ,P<0.05 both;(2) Compared with GK MI group ,there were significant reductions in optical density [ (0.84 ± 0.03) vs .(0.70 ± 0.03)] and mRNA ex-pression [ (0.64 ± 0.03) vs .(0.52 ± 0.03)] of Caspase-3 in GK MI + CoCl2 group ,P<0.05 both;(3) There were no significant difference in blood glucose level ,optical density and mRNA expression of Caspase-3 in Wistar MI group and Wistar MI + CoCl2 group , P>0.05 all .Conclusion:Chronic cobalt chloride treatment can decrease blood glucose level and reduce expression of Caspase-3 apoptosis gene in ischemic necrosis area in diabetic rats .
