Influence of cigarette smoking extract on fluorescence expression of thrombomodulin on surface of COS-7 cells
10.3969/j.issn.1008-0074.2015.03.04
- VernacularTitle:香烟提取物对 COS-7细胞表面血栓调节蛋白荧光表达的影响
- Author:
Yujie WEI
;
Huiliang LIU
;
Yi LI
;
Bin LAI
- Publication Type:Journal Article
- Keywords:
Tobacco;
Thrombomodulin;
COS cell;
Green fluorescent proteins
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2015;24(3):243-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether cigarette smoking extract (CSE) has influence on fluorescence protein ex-pression of thrombomodulin (TM) on surface of African green monkey kidney fibroblast cells (COS-7) or not . Methods:When TM-green fluorescent protein (GFP) plasmid was successfully constructed ,COS-7 cells were trans-fected by it ,then incubated by prepared 5% CSE (5% CSE group) ,PBS of certain volume was added to serum-free minimal essential medium (MEM ,simple medium) ,which was cultured at the same time and treated as control group .Flow cytometry counting method was used to detect change of TM-GFP expression amount on COS-7 surface at different time point .Results:Compare with control group ,there were no significant difference in the expression of TM-GFP on COS-7 surface at 1h and 6h in 5% CSE group [1h :(134.99 ± 18.41) vs .(146.61 ± 12.06) ,6h :(116.89 ± 27.28) vs .(123.89 ± 39.24) ,P>0.05 both] .Conclusion:The 5% cigarette smoking extract has no influ-ence on fluorescence expression of thrombomodulin on surface of African green monkey kidney fibroblast cells .
