Protection of the Extracts of Walnut kernel on Learning and Memory Abilities and Hippocampal Neuron in Rat Model of Alzheimer's Disease
10.3870/yydb.2015.06.005
- VernacularTitle:核桃仁提取物对阿尔茨海默病模型大鼠学习记忆能力和海马区神经元的保护作用
- Author:
Aini FU
;
Shuxiu ZHU
;
Yongxun AI
;
Qiang FU
- Publication Type:Journal Article
- Keywords:
Walnut kernel;
Alzheimer's disease;
Learning and memory ability;
Ultrastructure
- From:
Herald of Medicine
2015;(6):722-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the influence of three kinds of Walnut kernel extracts on learning and memory ability as well as ultrastructural pathology in hippocampus of Alzheimer's disease (AD) rats. Methods AD rat model was established by injection of amyloid-beta protein (Aβ1-40 ) into the nucleus basalis of meynert. The AD rats were randomly divided into Walnut kernel-water group,Walnut kernel-ethanol group,Walnut kernel-acetone group,and the model control group,10 rats in each group. In addition,10 rats of normal control group and 10 rats of sham operation group were selected. The model control group was not treated; the treatment groups were intragastrically given Walnut kernel water extract,ethanol extract,and acetone extract ( the equivalent of pharmacognosy 0. 3 g·mL-1 ),respectively,dose 3 g·kg-1 . The learning and memory ability was studied by Morris water maze,and ultrastructure of neurons was observed under the transmission electron microscopy. Results The time of looking for platform in Walnut kernel-water group,Walnut kernel-ethanol group,and Walnut kernel-acetone group were dropped swiftly at the beginning of 3 days, the third day is (51. 80±4. 37),(61. 20±4. 67),and (59. 63±5. 24),respectively; the model control group is (67. 67±6. 12) s. Compared with the model group,the differences were significance (P<0. 05); However,the acetone extract of Walnut kernel can obviously enhance the learning and memory ability (P<0. 01),and the ultrastructure almost returned to normal. Conclusion The acetone extracts of Walnut kernel have the function of preventing Alzheimer's disease.
