The influences of three methods on determination of salivary alpha-amylase activity and its activity ratio from the saliva before and after citric acid stimulation
10.3969/j.issn.1673-4130.2015.11.004
- VernacularTitle:3种方法对酸刺激前后唾液淀粉酶活性及其活性比值检测的影响
- Author:
Zemin YANG
;
Jing LIN
;
Xiaorong YANG
;
Longhui CHEN
;
Weiwen CHEN
- Publication Type:Journal Article
- Keywords:
method of iodine-starch;
method of Bernfeld;
method of EPS-G7 velocity;
salivary alpha amylase;
activity ratio
- From:
International Journal of Laboratory Medicine
2015;(11):1488-1490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differences of salivary alpha(α) amylase (sAA) activity and its activity ratio from the sali‐va before and after citric acid stimulation and approach the correlations among sAA activity determined by the methods of iodine‐starch ,Bernfeld and EPS‐G7 velocity respectively .Methods Ten saliva samples were collected from five healthy volunteers before and after citric acid stimulation .Their activities were determined three times by the three methods ,and the variation coefficient (CV) of sAA activity and activity ratio were calculated .Moreover ,correlation among sAA activities determined by the three meth‐ods were analyzed .Results The significant differences (P< 0 .05) were found in sAA activity and total CV from three determined methods ,and sAA activity and total CV by the method of EPS‐G7 velocity were minimum .There were no significant differences (P> 0 .05) in sAA activities ratio and its CV ;Significant correlation was found between sAA activity determined by random two of three methods(P < 0 .05) ,and their correlation coefficients were above 0 .96 .Conclusion The sAA activity data determined by three methods could be transformed each other by regression equation ,and determined precision by the method of EPS‐G7 velocity is highest ,and data processing method of sAA activity ratio could decrease differences among CV from three methods .
