Artemether inhibits proliferation and invasion via the mediation of peroxisome proliferator-activated ;receptor-gamma activation pathway in Lewis lung cancer cells
10.3760/cma.j.issn.1673-422X.2015.06.001
- VernacularTitle:蒿甲醚激活 PPARγ途径抑制小鼠 Lewis 肺癌细胞体外增殖和侵袭机制研究
- Author:
Fenglian FU
;
Yongxin JIANG
;
Yin CHENG
;
Shan LIU
;
Hong WANG
- Publication Type:Journal Article
- Keywords:
Artemisinins;
Lung neoplasms;
Peroxisome proliferators-activated receptors;
Receptor activator of nuclear factor-kappa B
- From:
Journal of International Oncology
2015;(6):401-406
- CountryChina
- Language:Chinese
-
Abstract:
mRNA in ARE and GW9662 group were 2.276 ±0.534 and 0.362 ±0.026,respectively.Compared with control group,PPARγmRNA level in both of ARE and GW9662 group reached statistical significance (t =4.785,P =0.001 ;t =2.395,P =0.044).PPARγprotein expression in ARE group,GW9662 +ARE group and control group were 27 688.33 ±3 593.06,21 816.00 ±1 644.07,17 716.33 ±2 273.95,respectively,which was higher in ARE group than that in control and GW+ARE group (t =5.159,P =0.001 ;t =3.038,P =0.016). NF-κB p65 mRNA expression in GW9662 +ARE group was 0.346 ±0.149,which in ARE group and GW9662 group were 0.392 ±0.1 87 and 1 .720 ±0.338,respec-tively.The differences of NF-κB p65 mRNA expression level between ARE,and control or GW9662 group were statistically significant (t =3.592,P =0.007;t =7.851 ,P =0.000).While,the differences of Caspase-3 mRNA and protein expression levels among the four groups were not statistically significant (F =1 .1 81 ,P =0.376;F =0.647,P >0.05).Conclusion ARE may restrain NF-κB through up-regulating PPARγto inhibit the proliferation and invasive potential of LLC in vitro, which suggests that PPAR-γmay be a novel therapeutic target for lung cancer.
