Determination of total glucoside in Paeonialactifloria Pall.by UV spectrophotometry
10.3760/cma.j.issn.1673-4246.2015.06.013
- VernacularTitle:紫外光谱法测定白芍药材中白芍总苷的含量
- Author:
Fen LIU
;
Congcong HOU
- Publication Type:Journal Article
- Keywords:
Paeonia lactifloriapall.;
UV spectrophotometry;
Total glucosides of paeony;
Quantitative determination
- From:
International Journal of Traditional Chinese Medicine
2015;(6):532-534
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveA method was proposed for the determination of the concent of total glucoside of peaony(TGP) fromPaeonia lactifloriaPall. by UV spectrophotometry.MethodsBased on the extract with 80% acidities alcohol, extracted by aether and n-butyl alcohol, TGP was obtained fromPaeonialactifloriaPall.. The determination was established with paeoniflorin which was the main component of TGP as a contrast by UV spectrophotometry at 230 nm.ResultsA good liner relationship between concentrationof paeoniflorin and its absorbance was obtained,and the liner range was between 0.040 2~0.201 1 mg/ml. The averagerecovery was 101.81%.ConclusionThis method is simple rapid and reliable. It could be used for the semi-quantitative determination of TGP of paeony in paeonialactifloria pall..
