Effects of Euphorbiae ebracteolatae Radixon the proliferation and apoptosis in human papillomavirus-18-infected HeLa cells
10.3760/cma.j.issn.1673-4246.2015.06.012
- VernacularTitle:中药狼毒对人乳头瘤病毒-18感染的HeLa细胞增殖及凋亡的影响
- Author:
Ping XU
;
Xiang HE
;
Xiaoying ZHANG
;
Huimin ZHANG
- Publication Type:Journal Article
- Keywords:
HeLa cells;
Human papillomavirus 18;
Euphorbiae ebracteolatae Radix;
Cell proliferation;
Apoptosis
- From:
International Journal of Traditional Chinese Medicine
2015;(6):529-531
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate effects ofEuphorbiae ebracteolatae Radixon the proliferation and apoptosis in human papillomavirus (HPV)-18-infected HeLa cells.MethodsThe alcohol extract ofEuphorbiae ebracteolatae Radixwas prepared. Cell proliferation was determined using the MTT assay, and apoptotic cells was determined using the Hoechst 33258 staining.ResultsHPV-18-infected HeLa cells were treated with 10, 20 μg/mlof the alcohol extract ofEuphorbiae ebracteolatae Radixfor 12 h, the cell proliferation inhibition rate were significantly increased compared to the control group (16.36% ± 5.06%, 18.59% ± 5.99%; allP<0.01);HPV-18-infected HeLa cells were treated with 0.625, 1.25, 2.5, 5, 10, 20 μg/ml of the alcohol extract ofEuphorbiae ebracteolatae Radixfor 72 h, the cell proliferation inhibition rate were significantly increased compared to the control group (13.82% ± 3.66%, 30.24% ± 6.58%, 43.29% ± 4.29%, 53.32% ± 1.41%, 54.24% ± 1.97%, 50.68% ±1.74%;allP<0.01). The Hoechst 33258 staining showed that HPV-18-infected HeLa cells were treated with 10 μg/ml of the alcohol extract ofEuphorbiae ebracteolatae Radixfor 24 h, the apoptotic cells were found.ConclusionEuphorbiae ebracteolatae Radixcould inhibit the proliferation and induce apoptosis in HPV-18-infected HeLa cells.
