The expression of BMP-2 mRNA of rat osteoblast cell sheets cultured with ascorbic acid
10.3969/j.issn.1001-3733.2015.03.01
- VernacularTitle:抗坏血酸对大鼠成骨细胞膜片 BMP-2 mRNA 表达的影响
- Author:
Chengju ZHOU
;
Jianjiang ZHOU
;
Hong YANG
;
Jiufeng MAO
;
Xiao ZENG
;
Yuanyuan JIA
;
Yuting WANG
;
Qiang DONG
- Publication Type:Journal Article
- Keywords:
Osteoblast cell sheets;
Ascorbic acid;
Bone morphogenetic protein-2(BMP-2)
- From:
Journal of Practical Stomatology
2015;(3):305-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of ascorbic acid on BMP-2 mRNA expression of osteoblast cell sheets.Methods:Rat os-teoblasts were primaryly cultured and identified;osteoblast cell sheets were built by physical scraping method in vitro;the osteoblast cell sheets were cultured with 1 5,50 and 85 mg/L ascorbic acid for 1 and 2 weeks respectively,and the expression of BMP-2 mRNA of the cell sheets was detected by RT-qPCR.Results:The obtained cells were conformed to be osteoblasts.The osteoblast cell sheets could be rolled into tube in vitro.The expression of BMP-2 mRNA of osteoblast cell sheets in experiment group,whether in week one or week two was higher than that in control group,50 mg/L group showed the highest expression(first week P <0.05;second week P>0.05);the expression of any group in week two was higher than that in week one(P <0.05).Conclusion:Ascorbic acid may pro-mote the expression of BMP-2 mRNA in osteoblast cell sheets.
