The effect of concentrate growth factor extract on MC3T3-E1 cells

VernacularTitle:浓缩生长因子提取液对 MC3T3-E1细胞影响的实验研究
Author: Lin LIU ; Kai DONG ; Zhonghao LIU
Publication Type:Journal Article
Keywords: Concentrate growth factors(CGF); Osseointegration; Osteoblast; Implant
From: Journal of Practical Stomatology 2015;(3):309-312
CountryChina
Language:Chinese
Abstract: Objective:To evaluate the effect of concentrate growth factor extract(CGFe)on the proliferation and differentiation of MC3T3-E1 osteoblasts.Methods:MC3T3-1 cells were cultured with and without CGFe respectively.At 1 ,3,5 d of culture the pro-liferation of the cells was detected by MTT assay,the activity of alkaline phosphatase (ALP)of the cells was examined by ALP kit;at 1 4,21 d of culture the calcium nodus formation was observed by Alizarin red staining;RUNX2 and OSX expression was quantified by real-time PCR.Results:With the extension of culture time,the proliferation,ALP activity,the calcium nodus and the level of RUNX2 and OSX mRNA of the experimental group were increased more than those of the control group.Conclusion:CGFe may pro-mote the proliferation and differentiation of osteoblasts.