Experimental study on the effect of RAMP1 -siRNA on CGRP-induced proliferation of MG-63 cells
10.3969/j.issn.1001-3733.2015.03.09
- VernacularTitle:RAMP1-siRNA 对 CGRP 促 MG-63细胞增殖作用影响的实验研究
- Author:
Zhang JIANG
;
Huiyu ZHANG
;
Gang ZHANG
;
Xiaoqian FENG
;
Song SUN
;
Yinghui TAN
- Publication Type:Journal Article
- Keywords:
Receptor activity modifying protein 1 (RAMP1 );
siRNA;
Calcitonin gene-related peptide(CGRP);
MG-63 cell;
Proliferation
- From:
Journal of Practical Stomatology
2015;(3):339-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of blocking the expression of receptor activity modifying protein 1 (RAMP1 )on calcito-nin gene-related peptide(CGRP)-induced MG-63 cell proliferation.Methods:RAMP1 siRNA was synthesized and screened by tran-scription in vitro.The subcultured MG-63 cells were divided into the following groups:RAMP1 siRNA interference group,empty vector group and blank control group.The mRNA expression and the membrane distribution changes of the calcitonin receptor-like receptor (CRLR)and the receptor component protein (RCP)in MG-63 cells were examined by real-time PCR and immunofluorescence method respectively.Results:RAMP1 and CRLR mRNA and the fluorescence intensity of MG-63 cells decreased after transfection by RAMP1 siRNA(P <0.05).In RAMP1 interference group,the expression of RCP mRNA and the fluorescence intensity were higher than those in the other two groups(P <0.05).After RAMP1 siRNA interference,the proliferation of MG-63 cells was inhibited(P <0.05). Conclusion:RAMP1 siRNA transfection may reduce CRLR expression and inhibite the proliferation of MG-63 cell.
