PLGA microsphere loading with PTH(1 -34)promotes osteogenesis:An in vitro study

VernacularTitle:负载 PTH（1-34）聚乳酸微球体外促成骨分化作用的研究
Author: Jingjing WANG ; Weiyan MENG ; Zhe ZHANG ; Yanmin ZHOU ; Shibo ZHANG ; Qing CAI ; Tongbin LIU ; Yue YUAN
Publication Type:Journal Article
Keywords: PTH(1 -34); PLGA; Controlled release
From: Journal of Practical Stomatology 2015;(3):360-364
CountryChina
Language:Chinese
Abstract: Objective:To determin the effect of PLGA microspheres loading with PTH(1 34)[PTH(1 34)/PLGA]on the differentiation of MC3T3E1 cells.Methods:MC3T3E1 cells were divided into control group,continuous or intermittent PTH(1 34)adminstration groups,PLGA microsphere group and PTH(1 34)/PLGA group.Osteogenesis differentiation was observed by alkaline phosphatase activity(ALP),alizarin red staining and RTPCR.Results:The PTH(1 34)/PLGA with 1 0 -9 mol/L final release concentration enhanced ALP activity and mineralization,increased the mRNA expression of RUNX2,ALP and VEGF.Conclusion:Controlledrelease of PTH(1 34)from PLGA microspheres can promote the osteogenesis differentiation of MC3T3E1 cells.