Quantitative evaluation of live Enterococcus faecalis in failed endodontic root-well-filled teeth
10.3969/j.issn.1001-3733.2015.03.015
- VernacularTitle:完善根管充填后治疗失败感染根管中粪肠球菌的活菌研究
- Author:
Fuhua ZHANG
;
Jianfeng ZHANG
;
Aimin HE
;
Zhijun WEI
- Publication Type:Journal Article
- Keywords:
Propidium monoazide(PMA);
Real-time polymerase chain reaction;
Root canal infection;
Entero-coccus faecalis(E.faecalis)
- From:
Journal of Practical Stomatology
2015;(3):365-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyse viable Enterococcus faecalis(E.faecalis)in root-well-filled teeth associated with failed endodontic treatment by using propidium monoazide (PMA)in combination with real time qPCR.Methods:Bacterial samples were extracted from 34 root-canal-treated teeth with post-treatment apical periodontitis.Each sample was separated into 2 different tubes.PMA was added to one of the tubes,and the other was left untreated.Then,DNA extraction and qPCR were performed.Results:E.faecalis was found in 20 of the 34 samples(58.8%).In PMA treated and none-treaten samples the Ct value of E.faecalis was 25.1 2 ±2.04 and 24.62 ± 2.02 respectively(P =0.001 ).Conclusion:PMA may be feasible in differentiating viable and dead Enterococcus faecalis cells.
