The relationship between plasma metabolic profiling and platelets activation on acute myocardium infarction patients
10.3760/cma.j.issn.1009-9158.2015.05.010
- VernacularTitle:急性心肌梗死患者血浆代谢谱与血小板激活的关系
- Author:
Chungang GU
;
Lei ZHANG
;
Hua KANG
;
Shuye LIU
- Publication Type:Journal Article
- Keywords:
Myocardium infarction;
Platelet activation;
Metabolomics
- From:
Chinese Journal of Laboratory Medicine
2015;(5):325-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identity the characteristic metabolites of platelets activation by Plasma metabolic Profiling in acute myocardium infarction ( AMI ) patients.Methods From August 2012 to February 2013, samples in three groups were collected at Tianjin Third Central hospital, including AMI group (25 clinically diagnosis myocardial infarction, 14 male, 11 female, average age 67 ±13 ) , control group(A) and simulation platelet activation group(B) (A and B group composed of 29 health volunteers, 11 male 18 female, average age 65 ±12 ) .After collagen platelet activation on B group, HPLC-LTQ Orbitrap XL MS platform was used to analyze the serum metabolic profiling in three groups respectively.Principal component analysis ( PCA) model and partial least squares-discriiminate analysis ( OPLS-DA) model were established to select characteristic metabolites in A and B group, and then tested in X group to find common ions.Results 20 characteristic metabolites were selected in A and B group.3 different lysophosphatidyl choline, sphingosine 1-phosphate, ethanol amine amides, sphingosine choline phosphate, thromboxane, 14-methyl hexadecanoic acid showed the same changing trend and were significant different between B group and AMI group.Conclusions Characteristic ions selected by metabolic profiling technology had significant distinguishing ability for AMI patients and health control.They may provide early diagnosis for AMI.
