Application of fluorescence resonance energy transfer technology in cancer research
- VernacularTitle:荧光共振能量转移技术在肿瘤研究中的应用进展
- Author:
Yu CHEN
;
Fangping XU
;
Yanhui LIU
- Publication Type:Journal Article
- Keywords:
Fluorescence resonance energy transfer;
Fluorescent protein;
Protein-protein interaction;
Neoplasms
- From:
Journal of Medical Postgraduates
2015;(6):661-665
- CountryChina
- Language:Chinese
-
Abstract:
In recent years , the emergence and progress of cancer genomics and targeted therapies have remarkably expanded the application fields of protein-protein interaction in cancer research .Fluorescence resonance energy transfer ( FRET ) is a kind of nonradiation energy transfer technique , which can timing , quantitative , positioning , and dynamically monitor the interaction between protein and protein in living cells .With the discovery of novel fluorescent proteins and the development of FRET -based biosensors , FRET has became an important method for visualizing spatial and temporal dynamics of interactions among biological macromolecules in native environments .This review summarises the recent studies and technological advances that have enhanced the use of FRET tech -nology in cancer basic research , early diagnosis , prognosis evaluation , drug development and other areas .
