Animal model construction of dog severe myocardial contusion and its application value

VernacularTitle:犬重度心肌挫伤动物模型的建立及其应用价值
Author: Weimin ZHANG ; Xingji ZHAO ; Wenhua DU ; Xiuqin XIONG
Publication Type:Journal Article
Keywords: myocardial contusion; hemorrhage; impact injury
From: Chongqing Medicine 2015;(15):2037-2039
CountryChina
Language:Chinese
Abstract: Objective To establish an animal model of dog severe myocardial contusion (MC) .Methods 12 dogs with the body weight of (11 .36 ± 1 .50)kg were selected .The BIM‐Ⅱ type biological impact machine was adopted to directly impact the bare area of the left chest by the driving pressure of 800 kPa ,the impact area was 16 .61 cm2 and the chest wall was inward compressed within 4-6 cm ,the severe myocardial contusion model was established .The pathological examination was taken at 8 h after injury and grading was performed according to the Abbreviated Injury Scale (AIS ,update in 2005) .Results The scattered large area bleeding spots appeared in the epicardium and endocardium of wound area .No obvious abnormality was found in the non - wound area .The pathological examination was performed based on the coronal plane of the left ventricular cross section .The percentage of the section 8 h necrosis area to the total area was (39 .78 ± 9 .07)% .Conclusion The established dog severe myocardial contusion model is stable and reproducible ,which can accurately simulate the temporary MC mode .