Methylation situation of let-7a-3 in chronic myeloid leukemia and its clinical significance
10.3969/j.issn.1671-8348.2015.15.002
- VernacularTitle:慢性髓系白血病中let-7a-3甲基化态势及其临床意义
- Author:
Dehong WU
;
Dongming YAO
;
Yun LI
;
Jiang LIN
;
Zhaoqun DENG
;
Jing YANG
;
Xingxing CHEN
;
Zhen QIAN
;
Jichun MA
;
Jun QIAN
- Publication Type:Journal Article
- Keywords:
chronic myeloid leukemia;
let-7a-3;
hypomethylation;
microRNA
- From:
Chongqing Medicine
2015;(15):2020-2023
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the methylation situation of let‐7a‐3 promoter in patients with chronic myeloid leukemia (CML) and its clinical significance .Methods The methylation level of let‐7a‐3 promoter in the bone marrow mononuclear cells of 52 CML patients and 25 controls was detected by using the real‐time quantitative methylation‐specific PCR (RQ‐PCR) .Results The non-hypomethylation of let‐7a‐3 promoter was positive in 31 cases(59 .6% ) of 52 CML patients ,while only 1 case(4% ,1/25) in the control group ,the difference between the two groups were statistically significant (P< 0 .01) .The ROC curve analysis showed that the non -hypomethylation of let‐7a‐3 has better specificity for the auxiliary diagnosis of CML .The significantly posi‐tive correlation was found between the non -methylation level of let‐7a‐3 promoter and the BCR/ABL transcription level (r=0 .641 ,P=0 .001) .In contrast ,there was no obvious correlation between the non -methylation level of let‐7a‐3 promoter and the WBC count ,platelet count and hemoglobin levels(P>0 .05) .The non-hypomethylation level of let‐7a‐3 in chronic phase and accel‐erate phase was significantly higher than that in blastic crisis of CML .Conclusion The hypomethylation level of let‐7a‐3 promoter is decreased with disease progression .
