Expression and clinical significance of microRNA-204 in human renal clear cell carcinoma
10.3969/j.issn.1671-8348.2015.15.006
- VernacularTitle:微小RNA204在人肾透明细胞癌中的表达及临床意义
- Author:
Linchao ZHANG
;
Junfeng ZHAO
;
Jijian SUN
- Publication Type:Journal Article
- Keywords:
mircoRNA-204;
renal clear cell carcinoma;
genes,Bcl-2
- From:
Chongqing Medicine
2015;(15):2034-2036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To inveatigate the expression and clinical significance of microRNA‐204(miR‐204) in human renal clear cell carcinoma(RCCC) .Methods 65 cases of resected RCCC tissue and corresponding normal tumor‐adjacent tissue were col‐lected and detected the expression level of miR‐204 by using qRT‐PCR .The correlation between the miR‐204 expression level and clilicalpathological features was statistically analyzed .The artificial miR‐204 mimics were adopted to stranfect into Caki‐1 cells ,then the Western blot was used to detect the expression of Bcl‐2 a as the downstream potential targets of miR‐204 and SIRT1 mRNA and protein .Results The expression level of miR‐204 in the RCCC tissue was significantly lowere than that in the normal tumor‐adjacent tissues (P<0 .05);the low expression of miR‐204 was significantly associated with the tumor size (>3 cm ,P<0 .05) , lymph node metastasis(P<0 .05) and advanced TNM stage(Ⅲ + Ⅳ ,P<0 .05) .Both the mRNA and protin expression in RCCC Caki‐1 cells were down‐regulated after transfection .Conclusion The expression of miR‐204 is down-regulated in the RCCC tissue and is related to the malignant clinicopathological features ,and miR‐204 may suppress the RCCC genesis and development through down‐regulating the expression of Bcl‐2 and SIRT1 .
