Effects of FSEER on immunosuppressive and bone-marrow-suppressive mice after chemotherapy
10.3969/j.issn.1000-484X.2015.05.010
- VernacularTitle:连翘根提取物（FSEER）对化疗后小鼠骨髓和免疫抑制的影响
- Author:
Jiawei SUN
;
Suli DAI
;
Lei LI
;
Xi YAN
;
Chao ZHANG
;
Lianmei ZHAO
;
Baoen SHAN
- Publication Type:Journal Article
- Keywords:
FSEER;
Cyclophosphamide( Cy);
Immunosuppression model;
Mice
- From:
Chinese Journal of Immunology
2015;(5):625-628
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of FSEER on the immunosuppressive and bone-marrow-suppressive mice after chemotherapy,and explore the mechanism of hematopoietic and immunologic function in mice accentuated by FSEER.Methods: Mice were injected cyclophosphamide(Cy)except control group,then randomly divided into mode group(saline),FSEER groups[120,60 mg/( kg· d) ].The spleen index( SI) of all mice was calculated respectively.Flow cytometry instrument testing mice peripheral blood lymphocytes CD3,CD4,CD8 change.The content of TNF-α,IL-2 and IL-12 in mice serum were measured by ELISA kits.Morphological images of bone marrow of the mice were observed under the microscope after Wright-Giemsa′s staining.Results:The spleen index( SI) was increased in both of the two FSEER groups.ELISA analyses showed that the content of TNF-αand IL-2 was increased in both of the two FSEER groups.The population of CD3+CD4+T lymphocyte and the ratio of CD4+/CD8+were all increased in the low dose experimental group.After treated with FSEER, the hematopoietic depression was improved significantly.Conclusion: FSEER can improve the state of immunosuppressive and myelosuppressive mice caused by Cy thus could alleviate the side effect of chemotherapy ef-fectively.
