Construction and identification of interference plasmid targeting on TNFAIP8
10.3969/j.issn.1000-484X.2015.05.015
- VernacularTitle:TNFAIP8干扰质粒的构建及最佳干扰效率质粒的筛选
- Author:
Wenming LIU
;
Jingjing YANG
;
Ruyi HU
;
Xingfeng QIU
;
Chunyan SHI
;
Zhongquan QI
;
Zhongchen LIU
;
Guohong ZHUANG
- Publication Type:Journal Article
- Keywords:
TNFAIP8;
RNA interference;
Recombinant plasmid;
Screen
- From:
Chinese Journal of Immunology
2015;(5):650-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-pSIREN-RetroQ.Methods:Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-pSIREN-RetroQ interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results:We successfully design and built three TNFAIP8-shRNA-pSIREN-RetroQ interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.
