Comparative study of bovine and porcine derived materials in hydrolysate samples by real-time fluorescence quantitative PCR and general PCR
- VernacularTitle:实时荧光定量PCR和常规PCR检测肝水解肽样品中牛、猪源性成分的对比研究
- Author:
Yan YU
;
Suting HE
;
Ziqiang WANG
;
Feng DENG
- Publication Type:Journal Article
- Keywords:
hydrolysate;
real-time fluorescence quantitative PCR;
general PCR;
bovine derived materials;
porcine derived materials
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(5):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare real-time fluorescence quantitative PCR with general PCR in detecting bovine and porcine derived materials in hydrolysate samples.Methods DNA were extracted from hydrolysate samples which prepared by different steps by real-time fluorescence quantitative PCR and general PCR.Results DNA of bovine and porcine could be detected by real-time fluorescence quantitative PCR and general PCR in samples prepared in the processes before enzymolysis solution, but not detected in samples from supermatant to the fourth ultrafiltrate.Conclusion Both real-time fluorescence quantitative PCR and general PCR can be applied to detect the fragments in hydrolysate samples.And real-time fluorescence quantitative PCR has the advantage such as rapid,convenient, non-environment-polluted, good repeatability, which improves the quality and efficiency.
