Comparative proteomic analysis of Yersinia pestis 201 and 201△pCD1 strains
10.7644/j.issn.1674-9960.2015.05.007
- VernacularTitle:鼠疫耶尔森菌201株和201△pCD1株蛋白表达谱比较研究
- Author:
Hongsheng LIU
;
Jingyu GUO
;
Erling FENG
;
Xiaoyi WANG
;
Hengliang WANG
;
Yajun SONG
;
Li ZHU
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
typeⅢsecretion system;
electrophoresis,gel,two-dimensional;
protein expression profiles;
mass spectrometry
- From:
Military Medical Sciences
2015;(5):348-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential pathogenesis of Yersinia pestis and provide new clues for vaccine development through comparative proteomic analysis of wild-type and pCD1 cured strain of Yersinia pestis 201.Methods Differentially expressed proteins at 26℃ and 37℃ were separated and identified using two-dimensional electrophoresis coupled with mass spectrometry .Results A total of 24 differently expressed proteins were successfully identified from the samples of bacteria grown at 26℃ and 25 proteins at 37℃.Among these, 7 proteins were encoded by pCD 1 plasmid. Conclusion Through comparative proteomic research, we have found that the abundance of several proteins can be dramatically changed when the large plasmid pCD 1 is missing,suggesting that the plasmid can regulate the expression of many genes located in the chromosome .
