Anticonvulsant effect of intranasal midazolam on maximal electroshock seizure and metrazol seizure model in rats
10.7644/j.issn.1674-9960.2015.05.012
- VernacularTitle:咪达唑仑经鼻给药在最大电休克发作及戊四唑惊厥模型的抗惊厥作用
- Author:
Kai TIAN
;
Yuan LUO
;
Wanhua LI
;
Xin SUI
;
Jun YANG
;
Aiping ZHENG
;
Peifeng WEI
;
Yongan WANG
- Publication Type:Journal Article
- Keywords:
midazolam;
diazepam;
seizure;
intranasal administration
- From:
Military Medical Sciences
2015;(5):368-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anticonvulsant effect of midazolam and diazepam when administered nasally on maximal electroshock seizure and metrazol seizure threshold test models .Methods Rats were randomly divided into 7 groups:model group, low-dose, middle-dose and high-dose of midazolam nasal spray groups , diazepam nasal spray in low-dose, middle-dose and high-dose of midazolam nasal spray groups .After the establishment of the maximal electroshock seizure( MES) and metrazol seizure threshold test models ( MST) in rats, the anticonvulsant effects of different doses of midazolam and the clinically used antiepileptic drug diazepam were evaluated and compared .HE staining was used to observe the histopathological changes in the hippocampus , cortex and amygdala in rats .Results Significant anticonvulsant effects were observed on MES and MST in rats pretreated with different dosages of midazolam .In addition , the anticonvul-sant effects of midazolam were stronger than those of diazepam at the same dosage on MES and MST (P<0.05,P<0.01). Histopathological results showed that both midazolam and diazepam could effectively prevent the seizure -induced brain inju-ries, inhibit the increase of microglial cells and the inflammatory cell infiltration in the hippocampus, cortex and amygdala, and reduce the nucleus pycnosis and neuronophagia .Conclusion Midazolam has significantly anticonvulsant and neuropro-tective effects on different seizure models when administered nasally in rats .
