Effect of inhibition of TGF-β1 receptors on lymphocytes during infection of Echi-nococcus granulosus
10.3969/j.issn.1000-484X.2015.05.007
- VernacularTitle:细粒棘球蚴感染中阻断TGF-β1受体对淋巴细胞的影响
- Author:
Shuanghong YIN
;
Junbo ZHANG
;
Xiaolin CHEN
;
Fangjie XU
;
Jun HOU
;
Xiangwei WU
;
Xueling CHEN
;
Yuanyong YAO
;
Mingming XING
;
Wencai YANG
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
T-lymphocyte subsets;
Treg cells;
NKG2D
- From:
Chinese Journal of Immunology
2015;(5):607-612
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of TGF-β1 on T lymphocytes of BALB/c mice infected with Echinococcus granulosus( E.granulosus ) in vitro.Methods: The inhibitor group:the spleen cells of BALB/c mouse were co-cultured with E.granulosus and SB525334.The control group:the spleen cells of BALB/c mouse were co-cultured with E.granulosus and PBS.The blank group:the spleen cells of BALB/c mouse were co-cultured with RPMI-1640 medium and SB525334.The lymphocytes were collected at 48 h post-infection.The T lymphocyte subsets, the number of CD4+CD25+T cells, the number of NK cells, and the expression of NKG2D receptor were detected by flow cytometry.The NK cell activity was determined with the lactate dehydrogenase leakage assay(LDH).Results:The inhibition the TGF-β1 receptors resulted in the increase of in the number of CD4+T cells,the decrease in the number of CD8+T cells,the increase of in the ratio CD4+/CD8+T cells,the decrease of in the number of CD4+CD25+T cells,the increase in the expression of the NKG2D receptors,the increase in the lysis rate of Yac-1 cells by NK cells,and a positive cor-relation between the expression of activity receptor NKG2D and killing activity of NK, which were mediated by E.granulosus.Conclusion: The inhibition of TGF-β1 receptors can enhance the immune response of T lymphocytes against E.granulosus infection in vitro.
