Study on working concentration of PDL in primary culture of rat hippocampal neuron
- VernacularTitle:多聚赖氨酸在大鼠海马神经元原代培养中工作浓度的研究
- Author:
Lu HAN
;
Feng XIAO
;
Xiumei ZHANG
- Publication Type:Journal Article
- Keywords:
hippocampal neurons;
primary culture;
working concentration of PDL
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(5):37-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore working of concentration of PDL used in primary culture of rat hippocampal neurons.Methods 3 pregnant wistar rats were executed by cervical dislocation, the embryos were taken out and the hippocampal tissue was dissected quickly.Then the tissue was digested by trypsin and planted into dishes with proper concentration(300000/3.5cm vessle) which were coated by different PDL solution in different concentration (0.01,0.05,0.1,0.25,0.5,0.75,1,2 mg/mL).The state of the cultured neurons was observed to determine the most suitable concentration of PDL solution in coating dishes.Neurons activity was observed by CCK-8 Kit.Results Most neurons had adhered in 4 h.Protrusion of neurons began to grow in 1 d.The connection between neurons appeared in 4 d.Neurons matured and the network-connection was set up in 7 d.The best working concentration of PDL is between 0.25 and 0.75 mg/mL, neurons grew well and activity was optimum during this concentration.Conclusion The working concentration of PDL is important for the hippocampal neuron culture and this work is worth being applied.
