Analysis of Piper puberulum (Benth.) Maxim.extracts preparation and the preliminary qualitative chemical composition
- VernacularTitle:黔产毛蒟提取物的制备及其化学成分的定性分析
- Author:
Yan YANG
;
Qin WU
;
Qihai GONG
;
Shibing XU
;
Jingshan SHI
- Publication Type:Journal Article
- Keywords:
Piper puberulum ( Benth.) Maxim.;
extraction process;
qualitative analysis;
orthogonal experiment design
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(5):169-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate optimal extraction process of Piper puberulum ( Benth.) Maxim.and qualitative analyze the chemical component of the extracts.Methods Method of solvent heating reflux was used for extraction.On the basis of single factor experiment, L9 (34 ) orthogonal experiment was designed with the variants of extraction frequency, time, material-liquid ratio, and immersion time.Extraction rate as index, extraction processes were optimized to achieve best extraction.The extracts, including total extract, water elution, and ethanol elution, were physiochemically analysed to achieve an initial qualitative result.Results The optimal extraction process was: extractions 3 times for 2 hours, with an 1︰30 material -liquid ratio and 2 hours of immersion, Initial qualitative analyzed the total extracts containing amino acids, polypeptides, proteins, alkaloids, steroids or triterpenes, flavones, saponins, polysaccharides, reducing sugars or glucosides, cumarins, terpene lactones, phenols, and tannins.The water elution containing: amino acids, polypeptides, proteins, saponins, polysaccharides, reducing sugars or glucosides, cumarins, and terpene lactones.The ethanol elution containing: amino acids, polypeptides, proteins, alkaloids, steroids or triterpenes, flavones, polysaccharides, reducing sugars or glucosides, phenols, and tanins.Conclusion The experiments show that optimal extraction process can achieve high extraction yield, stable and practical.
